Ligation Sequencing Kit V14 SQK-LSK114
A versatile sequencing kit optimised for modal raw read accuracy of Q20+ (99%+) and long read singleplex samples.
This uses our latest Kit 14 chemistry
- Preparation time: 60 minutes
- Read length: = fragment length
- Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR,100-200 fmol of amplicons or cDNA
Ligation Sequencing Kit XL V14 SQK-LSK114-XL
A versatile sequencing kit optimised for modal raw read accuracy of Q20+ (99%+) with long read singleplex samples. Multiple samples can be processed simultaneously.
This uses our latest Kit 14 chemistry
- Preparation time: 60 minutes
- Read length: = fragment length
- Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR, 100-200 fmol of amplicons or cDNA
Native Barcoding Kit 24 V14 SQK-NBD114.24
A versatile method of preparing barcoded sequencing libraries optimised for modal raw read accuracy of Q20+ (99%+) and long read multiplexed samples.
This uses our latest Kit 14 chemistry
- Preparation time: 140 minutes
- Read length: = fragment length
- Input amount: 400-1000 ng for gDNA. 200 fmol (130 ng for 1 kb amplicons) for amplicon input
Native Barcoding Kit 96 V14 SQK-NBD114.96
A versatile method of preparing barcoded sequencing libraries optimised for modal raw read accuracy of Q20+ (99%+) and long read multiplexed samples.
This uses our latest Kit 14 chemistry
- Preparation time: 140 minutes
- Read length: = to fragment length
- Input amount: 400 ng per sample of gDNA, 200 fmol per sample (130 ng for 1 kb amplicons)
Ultra-Long DNA Sequencing Kit V14 SQK-ULK114
An overnight method to prepare ultra-high molecular weight DNA for sequencing ultra-long reads (N50 >50 kb).
This is an Early Access product
This uses our latest Kit 14 chemistry
- Preparation time: ~200 minutes + overnight elution
- Read length: N50 >50 kb
- Input amount: 6 million cells
Rapid Barcoding Kit 24 V14 SQK-RBK114.24
Simple and rapid library preparation, with barcoding for up to 24 gDNA samples.
This uses our latest Kit 14 chemistry
- Preparation time: 60 minutes
- Read length: Random distribution, dependent on input fragment length
- Input amount: 200 ng dsDNA per sample
Rapid Barcoding Kit 96 V14 SQK-RBK114.96
Simple and rapid library preparation, with barcoding for up to 96 gDNA samples.
This uses our latest Kit 14 chemistry
- Preparation time: 60 minutes
- Read length: Random distribution, dependent on input fragment length
- Input amount: 200 ng dsDNA per sample
Rapid Sequencing Kit V14 SQK-RAD114
A simple and rapid library preparation method for genomic DNA optimised for speed with improved modal raw read accuracy.
This is an Early Access product
This uses our latest Kit 14 chemistry
- Preparation time: 10 minutes
- Read length: Random distribution, dependent on input fragment length
- Input amount: 100 ng dsDNA
Rapid PCR Barcoding Kit 24 V14 SQK-RPB114.24
Rapid and simple method of preparing up to 24 barcoded samples for sequencing from 1-5 ng of gDNA using PCR.
This is an Early Access product
This uses our latest Kit 14 chemistry
- Preparation time: 15 minutes + PCR
- Read length: ~2 kb
- Input amount: 1-5 ng gDNA per sample
16S Barcoding Kit 24 V14 SQK-16S114.24
Genus-level bacterial identification with barcoding for up to 24 samples.
Product lead time: 1 week
This uses our latest Kit 14 chemistry.
- Preparation time: 25 minutes + PCR
- Read length: Full-length 16S gene (~1.5 kb)
- Input amount: 10 ng gDNA per sample
Multiplex Ligation Sequencing Kit XL V14 SQK-MLK114.96-XL
A sequencing kit optimised for high output and PCR-free low-plex sequencing of 2-3 samples for Whole Genome Sequencing (WGS).
This uses our latest Kit 14 chemistry
Product lead time: 1 week
- Preparation time: 150 minutes
- Read length: = Fragment length
- Input amount: 1000 ng dsDNA per sample
Direct RNA Sequencing Kit SQK-RNA004
A sequencing kit optimised for sequencing native RNA with improved output and accuracy.
This is an Early Access product.
Product lead time: 1 week
- Preparation time: 135 minutes
- Read length: = RNA length
- Input amount: 1 μg total RNA, 300 ng poly(A)+ RNA
cDNA-PCR Sequencing Kit V14 SQK-PCS114
A sequencing kit optimised for identification and quantification of full-length transcripts with highest output.
This is an Early Access product
Product lead time: 6 weeks
This uses our latest Kit 14 chemistry.
- Preparation time: 225 minutes + PCR
- Read length: Enriched for full-length cDNA during PCR
- Input amount: 10 ng poly(A)+ RNA or 500 ng total RNA
cDNA-PCR Barcoding Kit V14 SQK-PCB114.24
A sequencing kit optimised for identification and quantification of full-length transcripts with highest output, including barcodes for up to 24 samples.
This is an Early Access product
Product lead time: 6 weeks
This uses our latest Kit 14 chemistry.
- Preparation time: 225 minutes + PCR
- Read length: Enriched for full-length cDNA during PCR
- Input amount: 10 ng poly(A)+ RNA or 500 ng total RNA per sample
COVID Maxi V14: 9216 samples C19MAXI114
A simple, scalable and rapid method for sequencing up to 9,216 SARS-CoV-2 samples using our V14 chemistry.
Product lead time: 1 week
COVID Midi V14: 2304 samples C19MIDI114
A simple, scalable and rapid method for sequencing up to 2,304 SARS-CoV-2 samples using our V14 chemistry.
Product lead time: 1 week
COVID Mini V14: 576 samples C19MINI114
A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples using our V14 chemistry.
Product lead time: 1 week
Flow Cell Wash Kit EXP-WSH004
Flow Cell Wash Kit XL EXP-WSH004-XL
The Flow Cell Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells on a higher scale.
This is compatible with all DNA sequencing kits.
Control Expansion Kit EXP-CTL001
RNA Control Expansion EXP-RCS001
RNA Control Sample to support control runs for cDNA kits.
This is an Early Access product
Flow Cell Priming Kit EXP-FLP004
Additional flow cell priming reagents for our V14 kits.
Note: This is only compatible with V14 chemistry
Flow Cell Priming Kit XL EXP-FLP004-XL
Additional flow cell priming reagents for our V14 kits.
Note: This is only compatible with V14 chemistry.
Sequencing Auxiliary Vials V14 EXP-AUX003
The Sequencing Auxiliary Vials contain Sequencing Buffer (SB), Elution Buffer (EB), Library Solution (LIS) and Library Beads (LIB) with V14 flow cell priming reagents: Flow Cell Flush (FCF) and Flow Cell Tether (FCT).
Note: This is only compatible with V14 chemistry.
Native Barcoding Auxiliary Kit V14 EXP-NBA114
Additional library preparation reagents for the Native Barcoding Kit 24 and 96 V14 (SQK-NBD114.24 and SQK-NBD114.96), and the Multiplex Ligation Sequencing Kit XL V14 (SQK-MLK114.96-XL).
Note: This is only compatible with V14 chemistry
Rapid Adapter Auxiliary V14 EXP-RAA114
Additional Rapid Adapter and Adapter Buffer to enable customers to maximise the use of our rapid sequencing chemistry kits.
Note: This is only compatible with V14 chemistry
EEB Expansion EXP-EEB001
Additional Extraction EB (EEB) for the Ultra-Long DNA Sequencing Kit V14 (SQK-ULK114).
Product lead time: 1 week
Note: This is only compatible with V14 chemistry.
Ultra-Long Auxiliary Vials EXP-ULA001
Additional library preparation reagents for the Ultra-Long DNA Sequencing Kit V14 (SQK-ULK114).
Product lead time: 1 week
Note: This is only compatible with V14 chemistry.
LFB Expansion EXP-LFB001
SFB Expansion EXP-SFB001
Short Fragment Eliminator Expansion EXP-SFE001
An expansion pack designed for size selection of high molecular weight DNA samples to progressively deplete short fragments of <25 kb.
This is compatible with all DNA sequencing kits.
PCR Expansion EXP-PCA001
PCR Barcoding Expansion 1-96 EXP-PBC096
The PCR Barcoding Expansion 1-96 (EXP-PBC096) is designed to allow the pooling and running together of multiple sequencing libraries.
- Preparation time: 150 minutes
- Read length: = to fragment length post PCR
- Input amount: 1000 ng dsDNA
PCR Barcoding Expansion 1-12 EXP-PBC001
The PCR Barcoding Expansion 1-12 (EXP-PBC001) allows the pooling and running together of multiple sequencing libraries.
- Preparation time: 150 minutes
- Read length: = to fragment length post PCR
- Input amount: 1000 ng dsDNA
Midnight RT PCR Expansion EXP-MRT001
An expansion kit containing all the required reagents and primers to generate reverse-transcribed and PCR-amplified material from SARS-CoV-2 RNA samples.
Product lead time: 1 week
This is compatible with Kit 10 and Kit 14 chemistry
- Preparation time: 80 minutes + PCR